Cowboy Junkies are an alternative country and folk rock band formed in Toronto, Ontario, Canada in 1985 by Alan Anton (bassist), Michael Timmins (songwriter, guitarist), Peter Timmins (drummer) and Margo Timmins (vocalist). The three Timminses are siblings, and Anton worked with Michael Timmins during their first couple of bands. John Timmins was a member of the band but left the group before the recording of their debut studio album. The band line-up has never changed since, although they use several guest musicians on many of their studio albums, including multi-instrumentalist Jeff Bird who has performed on every album except the first.

Cowboy Junkies' 1986 debut studio album, produced by Canadian producer Peter Moore, was the blues-inspired Whites Off Earth Now!!, recorded in the family garage using a single ambisonic microphone.

The band gained wide recognition with their second studio album, The Trinity Session (1988), recorded in 1987 at Toronto's Church of the Holy Trinity. Their sound, again with Peter Moore using the ambisonic microphone, and their mix of blues, country, folk, rock and jazz earned them both critical attention and a strong fan base. The Los Angeles Times named the recording one of the 10 best albums of 1988.

Cowboy Junkies have gone on to record 16 studio albums and five live albums, with tour dates booked into 2024.